| Distance | 62.0 mi |
| Distance (metric) | 99.8 km“100K - 4 loops + 6-mile start section / Total ≈ 62 miles” source |
| Course type | multi-loop“100k – 4 loops + starter loop” source |
| Net elevation | 0 ft“14.35-mile loop on scenic Wolf River trails” source |
| Nearest town | Memphis“Memphis, TN” source |
| State | TN“Memphis, TN” source |
| Country | US“Memphis, TN” source |
| Race starts at | Wolf River Refuge Trailhead, S. Walnut Bend Rd., Memphis, TN 38120“The Mamba 100 is held at the Wolf River Refuge Trailhead on S. Walnut Bend Rd., Memphis, TN 38120.” source |
| Date | 2026-11-06 |
| Start time | 19:00 |
| Entry fee | $215 |
| Aid stations | 12 |
| Drop bags | yes |
| Runners | 500 |